Ribosomal RNA (rRNA) makes up over 80–90% of total RNA in most eukaryotic and prokaryotic cells, significantly masking the detection of messenger RNA (mRNA) and non-coding RNAs in RNA sequencing.
Introduction to Magnetic Bead-Based Nucleic Acid Extraction Magnetic bead-based nucleic acid extraction has become a cornerstone technique in molecular biology due to its scalability, reproducibility, and compatibility with automation. Magnetic
Site-directed mutagenesis (SDM) is a foundational technique in modern molecular biology that enables the intentional alteration of DNA sequences at precise locations. It has become a cornerstone in applications such
The performance of PCR, one of the most essential tools in molecular biology, is heavily influenced by the chemical composition of its buffer system. While enzymes and primers often receive
A Practical Guide for Accurate Nucleic Acid Quantification Accurate and reliable quantification of nucleic acids is essential in modern molecular biology and genomic workflows. The Qubit™ Fluorometer from Thermo Fisher
Introduction Next-Generation Sequencing (NGS) has enabled researchers to explore genome-wide patterns with unprecedented precision. From base-level resolution to whole-genome comparisons, the demand for accuracy in sequencing outputs has never been
Newcastle Disease Virus (NDV) is a globally distributed, avian paramyxovirus that causes serious outbreaks in poultry populations. This RNA virus poses a major challenge to bird farms, aviaries, and hatcheries,
The Annexin A2 ELISA is widely used in biological and biochemical research to detect and measure the concentration of Annexin A2 (ANXA2) protein in samples such as serum, plasma, cell
The Anti-14-3-3 alpha+beta antibody is widely applied in laboratory protocols designed to identify and study 14-3-3 family proteins, a group of highly conserved regulatory molecules that bind to phosphorylated motifs
Introduction to Canine Sex Determination Sex determination in mammals is a tightly regulated process that begins early during embryogenesis. In dogs (Canis lupus familiaris), the presence or absence of a